Witzenhausen (German pronunciation:[vɪtsn̩ˈhaʊzn̩]) is a small town in the Werra-Meißner-Kreis in northeastern Hesse, Germany.

It was granted town rights in 1225, and until 1974 was a district seat.

The University of Kassel maintains a satellite campus in Witzenhausen, which offers an ecological agricultural sciences programme, putting Witzenhausen among Germany's smallest university towns. There is also a teaching institute (DEULA) for environment and technology, agriculture, horticulture and landscaping. The town is nationally known for the invention of the Biotonne biological refuse container, and is an important cherry-growing area, with a yearly Kesperkirmes or “Cherry Fair” (Kesper is a regional name for the cherry), at which a Cherry Queen (Kirschenkönigin) is chosen.